IVF PRO "Blastnaya" medium is developed for embryo cultivation from the morula stage to the blastocyst stage.
IVF PRO "Blastnaya" medium is used in assisted reproductive technologies, is intended for use in medical and preventive and research institutions.
The medium is prepared on the bicarbonate buffer basis.
Composition: purified water, mineral salts, carbohydrates, amino acids, vitamins, human serum albumin. Albumin, tested for presence of hepatitis B surface antigen, for antibodies to hepatitis C viruses, HIV-1 HIV-2, for antibodies to the causative agent of syphilis.
The medium can be prepared either with the addition of antibiotics and phenol red, or without these components (at customer’s request).
IVF PRO "Blastnaya" medium is sterilized by filtering through filters with pore size of 0.22 microns.
APPLICATION PROTOCOL
- Before use, the slightly opened vial with the medium is kept in atmosphere of 5-6% CO2 at temperature of 37 ° C for 2 hours
- Embryos at the morula stage (Day 3) are gently washed with balanced IVF PRO "Blastnaya" medium and transferred to a new portion of the same medium.
- Cultivation can be carried out in microdrops with medium coated with liquid paraffin oil, or in 500 ml of medium without oil coating.
- If it is necessary to transfer embryos to the uterus on Day4, medium IVF PRO "Shvartovaya" is used in a volume of 20-30 µl, pre-balancing it in atmosphere of 5-6% CO2 at temperature of 37 ° C for 2 hours.
Shelf life- 3 months from the date of manufacture, subject to the storage regime.
Store and transport in accordance with SR 3.3.2.1248-03 at temperature of 2 to 8 ° C in a place protected from light.
Do not freeze!
Unused residue of the heated medium is not subject to reheating and use.
The product must be used within 7 days from the bottle first opening.
It is recommended to store the medium with a minimum volume of air in the bottle.
Open the vial in sterile conditions.
